Revisiting an old paper, but a good one

I have to brag a bit in the title because if you say a paper is an “oldie,” you have to also say “it’s a goodie”. But I think this one is—it’s the first of two papers I wrote with my then-grad-student Allen Orr on the time course of speciation in Drosophila.  And it’s one of the few good ideas I’ve ever had. I don’t know how often it’s been cited—I don’t look up stuff like that—but it has been influential in inspiring others to do related work. I’m writing about this paper because I recently revisited it in an interview (see below).

Here’s a very brief summary of what we did. I realized one day, when I was at the University of Maryland, that there existed a tremendous amount of data about the sexual isolation and hybrid sterility/inviability of various Drosophila (fruit fly) species tested in the lab. There also existed, separately, a large amount of data on the “genetic distance” between these species as judged from gel electrophoresis. This genetic difference is a rough measure of the times since the species diverged. The more similar the electrophoretic profiles, the younger the species. (The actual real-time calibration of the distance is hard, as Drosophila has no fossil record, but we did our best.)

You could, I realized, take various pairs of species, see how much reproductive isolation they had between them—how much mating discrimination and whether the hybrids were viable and fertile—and correlate that with the genetic distance between members of each pair. If you plotted genetic distance against the degree of genetic isolation, you could get a “time course” of speciation, seeing which forms of isolation evolved earliest, what rate they evolved at, whether it would make a difference if the species lived in the same or different areas, and so on.

Of course there are lots of issues here, one being that measures of reproductive divergence between various pairs of species aren’t evolutionarily independent, so we had to do phylogenetic corrections. Further, sexual isolation and sterility/inviability are only two of the reproductive barriers that separate species, and we had to neglect types of genetic isolation that could operate in nature but couldn’t be measured in the lab (e.g., different preferences for food or microhabitats).

The results, though, were surprisingly clean and enlightening. For example, we found that sexual isolation—but not hybrid inviability—evolves ten times faster between species now found in the same area than those now found in different areas. This result, which has held up in repeats of our work, suggests that natural selection “reinforces”, or strengthens, mate discrimination between species when they live in the same place. That’s probably because there is a genetic penalty to be paid, in the form of hybrid problems, if you actually mate with the “wrong” species; and you only have that kind of selection operating in species that live in the same area, and have a chance to produce hybrids.

Here’s a graph from the second of our paper of papers showing two plots of the degree of sexual isolation between pairs of species (y axis) against their electrophoretic genetic distance (a measure of the divergence time between members of each pair). “Allopatric” taxa are pairs of species that are geographically isolated at present, while “sympatric” taxa are pairs of species that live in the same general area. (These data are phylogenetically corrected.) You can see that the degree of sexual isolation appears much earlier (at lower genetic distances) when the taxa live in the same area. This is a very striking result that is highly statistically significant. It suggests that natural selection operates on species living in the same place to “reinforce” their sexual isolation. You don’t see this difference for hybrid sterility or inviability, which are not expected to be reinforced by selection.

I digress, but it’s nice to think about this good old work.  Allen came on board the project at the beginning, and we spent several years collecting the data (which was scattered all over the literature), calculating statistics when only raw data were given, and analyzing the data. Thus the paper didn’t come out (in Evolution) until 1989, three years after we’d moved to Chicago.

Then electrophoretic data and reproductive-isolation data continued to accumulate, so in 1997 we published an update of the 1989 paper. The additional data confirmed the patterns we’d seen before. And now, since nobody does electrophoresis any more, and estimates of genetic divergence come from DNA sequences, we can’t do this analysis further. (DNA-sequence data does not exist for most of the species we used.)  Similar work has been done in fish and tomatoes, and at least two researchers have redone our analyses in flies using different techniques (the conclusions remain good).

The references to our two papers are given at the bottom, along with the links to them (free access).

This long introduction just wrote itself, when what I really want to do is call your attention to an interview I did about that first paper with Hari Sridhar at his site Reflections on Papers Past. Hari, a a post-doctoral researcher at the National Centre for Biological Sciences, Bengaluru (formerly Bangalore), India, has been interviewing scientists about well known papers in ecology and evolution since 2016.  He was kind enough to interview me about the first Coyne and Orr paper, and you can see the interview by clicking on the link below. I haven’t read the final version, which is a transcript of an audio conversation, so be aware that it’s spoken language. I did read a draft and corrected a few phrases that were unintelligible over the phone.

If you’re interested in papers in ecology and evolution, you might have a wander round Hari’s site; there are lots of interesting papers and interviews, many with people I know.

Click below to see the interview.

I want to add that although Allen was my grad student during much of the time we wrote these papers, it was a total collaboration. As with all my students, I don’t micromanage their work or ever tell them what research to do. Allen was interested in the project from the beginning, and contributed tons of work and many ideas to the two papers. And our collaboration continued in what I consider my most important scientific accomplishment, the book Speciation (Coyne and Orr, 2004; note that the book is now expensive but was about $50 when it first came out).

Here are Allen and I at the Evolution meetings in Portland in 2010. Allen was president of the Society for the Study of Evolution, and I was an incoming President, so he briefed me about the job. We had a great time in Portland, as that was before the city went nuts.

And as a measure of the fame of our work, you can’t get bigger than this. My collaboration with Allen was featured in the 2001 movie “Evolution” (a dreadful film!), as a scrawled reference on the blackboard behind two of the stars, David Duchovny and Orlando Jones. See below. It says “Read Coyne and Orr.  ‘Drosophila’ pp. xx8-450”. Note that the page numbers don’t correspond to either paper that we wrote, though it may refer to the book. But even in the book those pages don’t correspond to anything that would be a reading assignment.

Another ex-student of mine, Mohamed Noor, called me up and said he’d seen the movie and noticed a reference to our paper on the blackboard. I didn’t believe him, so I had to go see the movie myself. Sure enough, we were in there! Someone later sent me a screenshot (below).

I would call that real fame! Pity they got the page numbers wrong. I’ve always wondered who wrote that on the board and how they knew about our work.

_______________

Coyne, J. A. and H.A. Orr.  1989.  Patterns of speciation in Drosophila.  Evolution 43: 362-381.

Coyne, J. A., and H. A. Orr. 1997. “Patterns of speciation in Drosophila” revisited. Evolution 51:295-303.

27 Comments

  1. ursula goodenough
    Posted October 13, 2020 at 9:50 am | Permalink

    Patterns of speciation: 1381 citations,

    Revisited: 825 citations.

    BRAVO!

    And #1 has tons of citations in 2020 papers, so alive and kicking.

  2. Posted October 13, 2020 at 9:52 am | Permalink

    The movie reference couldn’t be to the book– the movie came out 3 years earlier.

    GCM

  3. ThyroidPlanet
    Posted October 13, 2020 at 10:06 am | Permalink

    Superficial comment :

    There’s a modern comic act, on Adult Swim which younger audiences – but not too young – probably know : Joe Pera. I perceive in the pic of PCC(E) leaning in – with a hip Hawaiian shirt (if that’s what they’re called) a distinct resemblance to … Joe Pera.

    • ThyroidPlanet
      Posted October 14, 2020 at 6:52 am | Permalink

      Here’s a photo of Joe Pera :

  4. Joseph McClain
    Posted October 13, 2020 at 10:42 am | Permalink

    Several years ago, I was art-directing a photo shoot at the math department of Prof. CC(E)’s alma mater. I asked an undergraduate to put some math on the blackboard by way of background. The department chair stepped up and warned the girl to be careful to write the correct math on the board.
    “We don’t want to be like what was on the windows in that John Nash movie, “A Beautiful Mind,” he said.
    “They had incorrect math in “A Beautiful Mind?” I asked.
    “Oh, yes,” he said. “Everybody laughed!”

  5. TJR
    Posted October 13, 2020 at 11:07 am | Permalink

    I was surprised by this aside

    “(DNA-sequence data does not exist for most of the species we used.)”

    Do you mean that we don’t have DNA sequence data for most Drosophila species? I naively thought that they must have been among the first species to be DNA sequenced.

    Why haven’t they been done, it seems very surprising?

    • Posted October 13, 2020 at 11:25 am | Permalink

      There are estimated to be 2500 species or so if you include the Hawaiians. Many of the species from which we got data aren’t in labs so much, and there’s no reason why people would want their sequences. And even for those that have been sequenced, people haven’t often calculated genetic distances between species pairs.

  6. Frank
    Posted October 13, 2020 at 11:47 am | Permalink

    I bought the (inexpensive-version) 2004 book as soon as it came out, and it was invaluable for modifying lectures on speciation in a basic evolution course. I also went through it with an Honors reading student.
    The book cut through a lot of the fog surrounding the discussion of sympatric speciation in animals at that time, much like Professor CCE’s useful dissection of the actual value and novelty of the punctuated equilibrium hypothesis.

  7. Posted October 13, 2020 at 12:03 pm | Permalink

    The Coyne-Orr paper and its followup work are true classics of evolutionary biology. There is one other case of a modern evolutionary biologist being referred to in a Hollywood movie. In the 1971 Elaine May / Walter Matthau movie A New Leaf a major development in the plot is when the Elaine May character discovers that a fern she discovered is a new species. She says “so I sent it to Wagner in Michigan and it’s a new species”. The reference is to the late Herb Wagner, a world expert on fern systematics who worked at the University of Michigan (and a really good guy). “Wagner parsimony” in reconstruction of phylogenies is named in his honor.

    • Posted October 13, 2020 at 1:30 pm | Permalink

      I will try to find that film – is it any cop? Also good to hear how our Dear Leader advanced knowledge of evolution 👍

      • Posted October 13, 2020 at 2:17 pm | Permalink

        (?) There are no cops in the film. It could have been a quirky and interesting film written and directed by Elaine May, but apparently Walter Matthau made demands and threatened to walk off the film, and so she had to give in, and Matthau ruined it enough that she does not even list it as one of hers. Nevertheless it is has its admirers.

      • dallos
        Posted October 14, 2020 at 4:24 am | Permalink

        • dallos
          Posted October 14, 2020 at 4:29 am | Permalink

          Sorry, something went wrong.
          [Youtube] Search for “Alsophila grahami”

    • Mike
      Posted October 13, 2020 at 3:05 pm | Permalink

      I love this kind of historical stuff. Like learning recently that the Brown in Brownian motion was a 19th C botanist who observed random motion of pollen grains in water. Einstein developed a model to account for Brown’s microscopic observations, and emphasized that it can’t be solved in terms of all the individual molecules, and only in probabilistic terms for the population of water molecules. And this became one of the important fundamental principles of population genetics (among other areas). I was slightly embarrassed to have done population genetics work for some time (and taught the subject) without knowing that historical detail.

      • Torbjörn Larsson
        Posted October 14, 2020 at 4:53 pm | Permalink

        Or, perhaps more important for physics and chemistry (and eventually biology), Einstein’s model became the conclusive test for atomic theory.

    • Torbjörn Larsson
      Posted October 14, 2020 at 4:56 pm | Permalink

      😀 I was going to comment on (Wagner) parsimony use and then I noted the comment author. [C.f. https://en.wikipedia.org/wiki/Maximum_parsimony_(phylogenetics) ]

      Thanks for the details!

  8. Nicolaas Stempels
    Posted October 13, 2020 at 12:52 pm | Permalink

    Clever research and really learning us something new (well at the time). Great!

  9. Posted October 13, 2020 at 1:21 pm | Permalink

    I liked the film! Daft but fun 😉 but then I have no tadte…

  10. Posted October 13, 2020 at 1:48 pm | Permalink

    Very good. I’ve added this to my notes for lectures on speciation. Although I do teach about how selection for reproductive isolation increases in sympatry, those graphs are especially clear presentations of the effect.

  11. Steve Pollard
    Posted October 13, 2020 at 3:36 pm | Permalink

    A great post, and a wonderful interview. Many thanks for both.

    PCC(E) comments: ‘useful data can be lying around in small, underappreciated journals’. I suspect this is the case in many other fields. When I was doing my inconsequential bit of post-graduate research, nearly 50 years ago now, I spent long hours in the library consulting the JCS, JACS, and indeed many smaller journals. I rather wish I had had the enterprise (and energy) to join some of the dots I spotted from time to time.

  12. darrelle
    Posted October 14, 2020 at 6:15 am | Permalink

    Looking forward to reading this on the weekend, when I can take the time to cogitate on it.

  13. Torbjörn Larsson
    Posted October 14, 2020 at 4:48 pm | Permalink

    Good idea indeed! And an interesting result.

    Time flies.

  14. uommibatto
    Posted October 16, 2020 at 4:07 pm | Permalink

    Cool story, though I struggled to understand it. Even so, it’s neat to read stuff like this.


Post a Comment

Required fields are marked *
*
*

%d bloggers like this: